scramble plko.1 shrna control  (Addgene inc)

Journal: iScience

Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment

doi: 10.1016/j.isci.2025.112198

Figure Lengend Snippet:

Article Snippet: Scramble pLKO.1 shRNA control (further termed “scramble”) was a gift from David Sabatini (Addgene plasmid #1864; http://n2t.net/addgene:1864; RRID:Addgene_1864). oeELF3 cells was generated by expressing human hELF3 (NM_004433) in pLenti-C-Myc-DDK-P2A-Puro (OriGene Technologies, Rockville, USA – CAT#: RC200631L3) and Luciferase (subcloned into pLenti-C-Myc-DDK-P2A-Puro expression vector). oeANXA4 cells were generated by expressing human ANXA4 (NM_001320698.2) in pcDNA3.1+/C-(K)-DYK vector (purchased from GenScript Biotech, Piscataway Township, USA – Clone-ID: OHu26294).

Techniques: Virus, Generated, Recombinant, Membrane, Electron Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Western Blot, RNA Sequencing, Gene Expression, Imaging, Plasmid Preparation, shRNA, Control, Luciferase, Cloning, Expressing, Software, Cell Analysis, Microscopy, Cell Culture

Journal: iScience

Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment

doi: 10.1016/j.isci.2025.112198

Figure Lengend Snippet:

Article Snippet: Scramble pLKO.1 shRNA control , Sarbassov et al. , Addgene plasmid #1864, RRID:Addgene_1864.

Techniques: Virus, Generated, Recombinant, Membrane, Electron Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Western Blot, RNA Sequencing, Gene Expression, Imaging, Plasmid Preparation, shRNA, Control, Luciferase, Cloning, Expressing, Software, Cell Analysis, Microscopy, Cell Culture